Sperm sorting is a gender-selection technique that improves a couple's chances of choosing their embryo's sex. A couple may choose this option to prevent certain genetic diseases or to promote family balancing. Prior to insemination of the egg, sperm sorting separates X-chromosome female sperm from Y-chromosome male sperm, both of which determine the sex of the offspring. This distinguishes sperm sorting from PGD, in which a fertilized embryo is chosen for transferal to the uterus based on its sex.
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Flow cytometric sorting can be used to separate sperm based on sex chromosome content. Differential fluorescence emitted by stained X- vs. Y-chromosome-bearing sperm enables sorting and collection of samples enriched in either X- or Y-bearing sperm for use to influence the likelihood that the offspring will be a particular sex. Herein we report the effectiveness of flow cytometric sorting of human sperm and its use in human ART procedures.
Sperm sorting is a means of choosing what type of sperm cell is to fertilize the egg cell. Several conventional techniques of centrifugation or swim-up. Newly applied methods such as flow cytometry expand the possibilities of sperm sorting and new techniques of sperm sorting are being developed.